The Western blot workflow for immuno-based protein detection was introduced over 30 years ago. Since its introduction, Western blotting has become a well-known and widely accepted technique routinely ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

An interview with Ramya Prabakaran & Melody Chang from GenScript, at SfN 2018, discussing the major challenges for researchers carrying out western blotting, and how GenScript's automated systems are ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

Western blotting can often be more trouble than expected. While SDS-PAGE and western blotting protocols are straightforward, there are many critical decision points that lead to success or failure. In ...

Discover how decisions about experimental design can affect Western blotting reproducibility – and how to make the right decisions in this webinar from Sally Mulford, Sr Molecular Biologist at LI-COR ...

It takes time to get the hang of new immunodetection methods, and during this learning period you may well see your share of failed experiments. Christiana Binkley, a PhD student in cellular and ...

GE Healthcare Life Sciences recently launched the Amersham WB system for quantitative SDS-PAGE and Western blotting of proteins using fluorescence detection. The system was standardized for the entire ...